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Simply and effectively preparing high-purity phosvitin using polyethylene glycol and anion-exchange chromatography.
- Source :
-
Journal of separation science [J Sep Sci] 2011 Nov; Vol. 34 (22), pp. 3295-301. Date of Electronic Publication: 2011 Oct 14. - Publication Year :
- 2011
-
Abstract
- An attempt was made to develop a new protocol for preparing phosvitin that could be easy scaled up using polyethylene glycol (PEG6000). Influence of PEG6000 concentration and pH of sample solution on phosvitin isolation was investigated. Phosvitin of high purity (99%) was obtained in good yield (47%) with the optimal condition of pH 4.0 and 3% PEG6000 precipitation. In addition, through evaluating different anion-exchange chromatography methods, the DEAE procedure at pH 7.5 was finally selected as the best procedure to obtain metal-free phosvitin that lost the least protein. Furthermore, it is observed that the purity and characterizations of prepared phosvitin were similar to those of the standard phosvitin from Sigma, and the random coil of phosvitin converted into more compact structure after removing metal ion. In conclusion, the developed method was simple and suitable for scaled up preparation of phosvitin.<br /> (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Details
- Language :
- English
- ISSN :
- 1615-9314
- Volume :
- 34
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Journal of separation science
- Publication Type :
- Academic Journal
- Accession number :
- 21998042
- Full Text :
- https://doi.org/10.1002/jssc.201100601