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Efficacy of IgG and F(ab')2 antivenoms to neutralize snake venom-induced local tissue damage as assessed by the proteomic analysis of wound exudate.
- Source :
-
Journal of proteome research [J Proteome Res] 2012 Jan 01; Vol. 11 (1), pp. 292-305. Date of Electronic Publication: 2011 Nov 15. - Publication Year :
- 2012
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Abstract
- Proteomic analysis of wound exudates represents a valuable tool to investigate tissue pathology and to assess the therapeutic success of various interventions. In this study, the ability of horse-derived IgG and F(ab')(2) antivenoms to neutralize local pathological effects induced by the venom of the snake Bothrops asper in mouse muscle was investigated by the proteomic analysis of exudates collected in the vicinity of affected tissue. In experiments involving the incubation of venom and antivenom prior to injection in mice, hemorrhagic activity was completely abolished and local muscle-damaging activity was significantly reduced by the antivenoms. In these conditions, the relative amounts of several intracellular and extracellular matrix proteins were reduced by the action of antivenoms, whereas the relative amounts of various plasma proteins were not modified. Because not all intracellular proteins were reduced, it is likely that there is a residual cytotoxicity not neutralized by antivenoms. In experiments designed to more closely reproduce the actual circumstances of envenoming, that is, when antivenom is administered after envenomation, the number of proteins whose amounts in exudates were reduced by antivenoms decreased, underscoring the difficulty in neutralizing local pathology due to the very rapid onset of venom-induced pathology. In these experiments, IgG antivenom was more efficient than F(ab')(2) antivenom when administered after envenomation, probably as a consequence of differences in their pharmacokinetic profiles.
- Subjects :
- Animals
Antivenins therapeutic use
Blood Proteins metabolism
Extracellular Matrix Proteins metabolism
Hemorrhage chemically induced
Hemorrhage prevention & control
Horses
Immunoglobulin Fab Fragments therapeutic use
Immunoglobulin G
Mice
Muscle, Skeletal drug effects
Muscle, Skeletal pathology
Proteomics
Antivenins pharmacology
Bothrops
Crotalid Venoms immunology
Exudates and Transudates metabolism
Immunoglobulin Fab Fragments pharmacology
Proteome metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 22004524
- Full Text :
- https://doi.org/10.1021/pr200847q