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Counting proteins bound to a single DNA molecule.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Nov 11; Vol. 415 (1), pp. 131-4. Date of Electronic Publication: 2011 Oct 12. - Publication Year :
- 2011
-
Abstract
- Chromosomes contain DNA covered with proteins performing functions such as architectural organization and transcriptional regulation. The ability to count the number of proteins bound to various regions of the genome is essential for understanding both architectural and regulatory functions. We present a straightforward method of counting gfp-conjugated proteins bound to an individual duplex DNA molecule by calibrating to a commercially available fluorescence standard using wide-field fluorescence microscopy. We demonstrate our method using the E. coli nucleoid-associated protein Fis.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- DNA metabolism
DNA-Binding Proteins chemistry
DNA-Binding Proteins metabolism
Escherichia coli Proteins analysis
Escherichia coli Proteins chemistry
Escherichia coli Proteins metabolism
Factor For Inversion Stimulation Protein analysis
Factor For Inversion Stimulation Protein chemistry
Factor For Inversion Stimulation Protein metabolism
Microscopy, Fluorescence standards
DNA chemistry
DNA-Binding Proteins analysis
Microscopy, Fluorescence methods
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 415
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 22020072
- Full Text :
- https://doi.org/10.1016/j.bbrc.2011.10.029