Back to Search
Start Over
Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil.
- Source :
-
Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2011 Oct; Vol. 44 (5), pp. 631-2. - Publication Year :
- 2011
-
Abstract
- Introduction: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE).<br />Methods: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program.<br />Results: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%.<br />Conclusions: RT-PCR provides same day results, however it showed low sensitivity for VRE detection.
- Subjects :
- Enterococcus isolation & purification
Gram-Positive Bacterial Infections microbiology
Humans
Predictive Value of Tests
Real-Time Polymerase Chain Reaction methods
Sensitivity and Specificity
Bacterial Proteins genetics
Carbon-Oxygen Ligases genetics
Enterococcus genetics
Gram-Positive Bacterial Infections diagnosis
Rectum microbiology
Vancomycin Resistance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1678-9849
- Volume :
- 44
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Revista da Sociedade Brasileira de Medicina Tropical
- Publication Type :
- Academic Journal
- Accession number :
- 22031081
- Full Text :
- https://doi.org/10.1590/s0037-86822011000500021