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A combination of resveratrol and melatonin exerts chemopreventive effects in N-methyl-N-nitrosourea-induced rat mammary carcinogenesis.
- Source :
-
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) [Eur J Cancer Prev] 2012 Mar; Vol. 21 (2), pp. 163-70. - Publication Year :
- 2012
-
Abstract
- The neurohormone melatonin is primarily involved in the regulation of circadian rhythms, but also acts as an antioxidant and anticarcinogenic agent, especially in breast cancer. Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a widely known polyphenolic agent from red wine, which has been shown to exert antioxidant, anti-inflammatory and anticarcinogenic effects. The objective of this study was therefore to investigate the effects of melatonin in combination with resveratrol in a rat model of experimental mammary carcinogenesis. Female Sprague-Dawley rats aged 31 days were used in the experiment. Mammary carcinogenesis was induced by N-methyl-N-nitrosourea (NMU), which was administered in two intraperitoneal doses (50 mg/kg of body weight). Chemoprevention with resveratrol and melatonin started 2 weeks before the first dose of NMU and lasted until the end of the experiment. The basic parameters evaluated were: tumour incidence, latency period, tumour frequency per group and tumour volume. In addition, oestrogen receptors ERα and ERß, melatonin receptor MT1, proliferating cell nuclear antigen and vascular endothelial growth factor were determined by immunohistochemical staining. The combination of resveratrol and melatonin reduced tumour incidence by approximately 17% and significantly decreased the quantity of invasive and in-situ carcinomas. Food intake declined in the second and seventh weeks after the administration of carcinogen. Resveratrol in combination with melatonin returned food intake to the level of intact controls. Resveratrol in combination with melatonin has some protective effects on NMU-induced rodent breast cancer. Further studies are necessary to confirm these effects of this promising combination.
- Subjects :
- Algorithms
Animals
Carcinogens
Carcinoma blood
Carcinoma chemically induced
Carcinoma pathology
Drug Evaluation, Preclinical
Estrogen Receptor alpha metabolism
Female
Mammary Neoplasms, Experimental blood
Mammary Neoplasms, Experimental chemically induced
Mammary Neoplasms, Experimental pathology
Melatonin blood
Methylnitrosourea
Proliferating Cell Nuclear Antigen metabolism
Rats
Rats, Sprague-Dawley
Resveratrol
Vascular Endothelial Growth Factor A metabolism
Antineoplastic Combined Chemotherapy Protocols therapeutic use
Carcinoma prevention & control
Chemoprevention methods
Mammary Neoplasms, Experimental prevention & control
Melatonin administration & dosage
Stilbenes administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1473-5709
- Volume :
- 21
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)
- Publication Type :
- Academic Journal
- Accession number :
- 22044852
- Full Text :
- https://doi.org/10.1097/CEJ.0b013e32834c9c0f