Distribution pattern of Plasmodium falciparum chloroquine transporter (pfcrt) gene haplotypes in Sri Lanka 1996-2006.

Authors :: Zhang JJ
Senaratne TN
Daniels R
Valim C
Alifrangis M
Amerasinghe P
Konradsen F
Rajakaruna R
Wirth DF
Karunaweera ND
Source :: The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2011 Nov; Vol. 85 (5), pp. 811-4.
Publication Year :: 2011
Abstract: Widespread antimalarial resistance has been a barrier to malaria elimination efforts in Sri Lanka. Analysis of genetic markers in historic parasites may uncover trends in the spread of resistance. We examined the frequency of Plasmodium falciparum chloroquine transporter (pfcrt; codons 72-76) haplotypes in Sri Lanka in 1996-1998 and 2004-2006 using a high-resolution melting assay. Among 59 samples from 1996 to 1998, we detected the SVMNT (86%), CVMNK (10%), and CVIET (2%) haplotypes, with a positive trend in SVMNT and a negative trend in CVMNK frequency (P = 0.004) over time. Among 24 samples from 2004 to 2006, we observed only the SVMNT haplotype. This finding indicates selection for the SVMNT haplotype over time and its possible fixation in the population.

Subjects :: Animals
Drug Resistance genetics
Haplotypes
Humans
Malaria, Falciparum epidemiology
Plasmodium falciparum drug effects
Sri Lanka epidemiology
Time Factors
Antimalarials pharmacology
Chloroquine pharmacology
Malaria, Falciparum parasitology
Membrane Transport Proteins genetics
Membrane Transport Proteins metabolism
Plasmodium falciparum genetics
Protozoan Proteins genetics
Protozoan Proteins metabolism

Full Text Access

Tools