Back to Search
Start Over
Impact of epiphytic and endogenous enzyme activities of senescent maize leaves and roots on the soil biodegradation process.
- Source :
-
Comptes rendus biologies [C R Biol] 2011 Nov; Vol. 334 (11), pp. 824-36. Date of Electronic Publication: 2011 Sep 07. - Publication Year :
- 2011
-
Abstract
- This study was focused on investigating the role of the initial residue community, i.e. microorganisms and enzymes from the epiphytic and endophytic compartments, in soil decomposition processes. Aerial and underground parts (leaves and roots) of maize (Zea mays L.) plants were γ-irradiated, surface-sterilized with sodium hypochlorite (NaOCl)/ethanol or non-sterilized (controls), while the outer surface morphology of maize leaves and roots was examined by scanning electron microscopy (SEM). Non-sterilized and sterilized maize leaves and roots were incubated in soil to study carbon (C) mineralization kinetics and enzyme dynamics (L-leucine aminopeptidase, CBH-1, xylanase, cellulase and laccase). SEM results showed that initial microbial colonization was more pronounced on non-sterilized leaf and root surfaces than on sterilized samples. The hypochlorite treatment removed a part of the soluble components of leaves by washing and no specific effect of any type of colonizing microorganisms was observed on C mineralization. In contrast, γ irradiation and hypochlorite treatments did not affect root chemical characteristics and the quantitative effect of initial residue-colonizing microorganisms on C mineralization was demonstrated. The variations in C mineralization and enzyme dynamics between non-sterilized and sterilized roots suggested that activities of epiphytic and endogenic microorganisms were of the same order of magnitude.<br /> (Copyright © 2011 Académie des sciences. Published by Elsevier SAS. All rights reserved.)
- Subjects :
- Analysis of Variance
Carbohydrates analysis
Cell Wall chemistry
Cellulase analysis
Colorimetry
Fluorometry
Gamma Rays
Kinetics
Lignin analysis
Microscopy, Electron, Scanning
Plant Leaves enzymology
Plant Leaves ultrastructure
Plant Roots enzymology
Plant Roots ultrastructure
Soil Microbiology
Sterilization
Biodegradation, Environmental
Enzymes chemistry
Soil analysis
Zea mays chemistry
Zea mays enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1768-3238
- Volume :
- 334
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Comptes rendus biologies
- Publication Type :
- Academic Journal
- Accession number :
- 22078739
- Full Text :
- https://doi.org/10.1016/j.crvi.2011.07.004