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Application of hollow fiber-supported liquid-phase microextraction coupled with HPLC for the determination of guaifenesin enantiomer-protein binding.
- Source :
-
Biomedical chromatography : BMC [Biomed Chromatogr] 2012 Jul; Vol. 26 (7), pp. 875-80. Date of Electronic Publication: 2011 Nov 20. - Publication Year :
- 2012
-
Abstract
- A hollow fiber liquid-phase microextraction technique coupled with high-performance liquid chromatography with fluorescence detection was employed for determination and evaluation of the binding characteristics of drugs to bovine serum albumin (BSA). Enantiomers of guaifenesin (an expectorant drug) were investigated as a model system. After optimization of some influencing parameters on microextraction, the proposed method was used for calculation of the target drug distribution coefficient between n-octanol and the buffer solution as well as study of drug-BSA binding in physiological conditions. The developed method shows a new, improved and simple procedure for determination of free drug concentration in biological fluids and the extent of drug-protein binding.<br /> (Copyright © 2011 John Wiley & Sons, Ltd.)
- Subjects :
- 1-Octanol chemistry
Animals
Cattle
Limit of Detection
Linear Models
Models, Chemical
Osmolar Concentration
Protein Binding
Reproducibility of Results
Stereoisomerism
Chromatography, High Pressure Liquid methods
Guaifenesin chemistry
Guaifenesin metabolism
Liquid Phase Microextraction methods
Serum Albumin, Bovine metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0801
- Volume :
- 26
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biomedical chromatography : BMC
- Publication Type :
- Academic Journal
- Accession number :
- 22102436
- Full Text :
- https://doi.org/10.1002/bmc.1746