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Acute renal venous obstruction is more detrimental to the kidney than arterial occlusion: implication for murine models of acute kidney injury.
- Source :
-
American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2012 Mar 01; Vol. 302 (5), pp. F519-25. Date of Electronic Publication: 2011 Nov 23. - Publication Year :
- 2012
-
Abstract
- In this study, we compared the traditional murine model with renal pedicle clamp with models that clamped the renal artery or vein alone as well as to a whole body ischemia-reperfusion injury (WBIRI) model. Male C57BL/6J mice underwent either clamping of the renal artery, vein, or both (whole pedicle) for 30 or 45 min followed by reperfusion, or 10 min of cardiac arrest followed by resuscitation up to 24 h. After 30 min of ischemia, the mice with renal vein clamping showed the mostly increased serum creatinine and the most severe renal tubule injury. After 45 min of ischemia, all mice with renal vasculature clamping had a comparable increase in serum creatinine but the renal tubule injury was most severe in renal artery-clamped mice. Renal arterial blood flow was most decreased in mice with a renal vein clamp compared with a renal artery or pedicle clamp. A 30-or 45-min renal ischemia time led to a significant increase in the protein level of interleukin-6, keratinocyte-derived chemokine (KC), and granular colony-stimulating factor in the ischemic kidney, but the KC was the highest in the renal pedicle-clamped kidney and the lowest in the renal vein-clamped kidney. Of note, 10 min of WBIRI led to kidney dysfunction and structural injury, although less than longer time clamping of isolated renal vasculature. Our data demonstrate important differences in ischemic AKI models. Understanding these differences is important in designing future experimental studies in mice as well as clinical trials in humans.
- Subjects :
- Acute Kidney Injury metabolism
Animals
Blood Flow Velocity
Chemokines metabolism
Cytokines metabolism
Disease Models, Animal
Ischemia metabolism
Kidney metabolism
Kidney physiopathology
Kidney Function Tests
Male
Mice
Renal Artery metabolism
Renal Artery Obstruction metabolism
Renal Veins metabolism
Acute Kidney Injury physiopathology
Ischemia physiopathology
Kidney blood supply
Renal Artery physiopathology
Renal Artery Obstruction physiopathology
Renal Veins physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1466
- Volume :
- 302
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Renal physiology
- Publication Type :
- Academic Journal
- Accession number :
- 22114209
- Full Text :
- https://doi.org/10.1152/ajprenal.00011.2011