Back to Search
Start Over
Crosstalk between integrin αvβ3 and estrogen receptor-α is involved in thyroid hormone-induced proliferation in human lung carcinoma cells.
- Source :
-
PloS one [PLoS One] 2011; Vol. 6 (11), pp. e27547. Date of Electronic Publication: 2011 Nov 22. - Publication Year :
- 2011
-
Abstract
- A cell surface receptor for thyroid hormone that activates extracellular regulated kinase (ERK) 1/2 has been identified on integrin αvβ3. We have examined the actions of thyroid hormone initiated at the integrin on human NCI-H522 non-small cell lung carcinoma and NCI-H510A small cell lung cancer cells. At a physiologic total hormone concentration (10(-7) M), T(4) significantly increased proliferating cell nuclear antigen (PCNA) abundance in these cell lines, as did 3, 5, 3'-triiodo-L-thyronine (T(3)) at a supraphysiologic concentration. Neutralizing antibody to integrin αvβ3 and an integrin-binding Arg-Gly-Asp (RGD) peptide blocked thyroid hormone-induced PCNA expression. Tetraiodothyroacetic acid (tetrac) lacks thyroid hormone function but inhibits binding of T(4) and T(3) to the integrin receptor; tetrac eliminated thyroid hormone-induced lung cancer cell proliferation and ERK1/2 activation. In these estrogen receptor-α (ERα)-positive lung cancer cells, thyroid hormone (T(4)>T(3)) caused phosphorylation of ERα; the specific ERα antagonist ICI 182,780 blocked T(4)-induced, but not T(3)-induced ERK1/2 activation, as well as ERα phosphorylation, proliferating-cell nuclear antigen (PCNA) expression and hormone-dependent thymidine uptake by tumor cells. Thus, in ERα-positive human lung cancer cells, the proliferative action of thyroid hormone initiated at the plasma membrane is at least in part mediated by ERα. In summary, thyroid hormone may be one of several endogenous factors capable of supporting proliferation of lung cancer cells. Activity as an inhibitor of lung cancer cell proliferation induced at the integrin receptor makes tetrac a novel anti-proliferative agent.
- Subjects :
- Cell Line, Tumor
Cell Nucleus drug effects
Cell Nucleus metabolism
Cell Proliferation drug effects
Enzyme Activation drug effects
Estradiol analogs & derivatives
Estradiol pharmacology
Extracellular Signal-Regulated MAP Kinases
Fulvestrant
Humans
Lung Neoplasms enzymology
Phosphorylation drug effects
Proliferating Cell Nuclear Antigen metabolism
Protein Transport drug effects
Thyroxine analogs & derivatives
Thyroxine pharmacology
Estrogen Receptor alpha metabolism
Integrin alphaVbeta3 metabolism
Lung Neoplasms metabolism
Lung Neoplasms pathology
Signal Transduction drug effects
Thyroid Hormones pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 6
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22132110
- Full Text :
- https://doi.org/10.1371/journal.pone.0027547