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Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase.
- Source :
-
Plant cell reports [Plant Cell Rep] 2012 Apr; Vol. 31 (4), pp. 737-45. Date of Electronic Publication: 2011 Dec 02. - Publication Year :
- 2012
-
Abstract
- Mitogen-activated protein kinases (MAPKs or MPKs) are one of the most important and conserved signaling molecules in plants. MPKs can directly modulate gene expression by the phosphorylation of transcription factors. However, only a few target substrates of MPKs have been isolated. Here, we identified a C(2)H(2)-type zinc finger transcription factor from Arabidopsis, ZAT10, as a substrate of MPKs. Using in vitro and in vivo protein-protein interaction analyses, we demonstrated that ZAT10 directly interacted with MPK3 and MPK6. ZAT10 was phosphorylated by recombinant Arabidopsis MPK3 and MPK6 in a kinase assay. Furthermore, ZAT10 was also phosphorylated by native MPK3 and MPK6 prepared from Arabidopsis plants in an in-gel kinase assay. Mass spectrometry analysis of phosphopeptides was used to determine two MPK phosphorylation sites in ZAT10. These sites were verified by site-directed mutagenesis and in vitro kinase assays.<br /> (© Springer-Verlag 2011)
- Subjects :
- Amino Acid Sequence
Arabidopsis metabolism
Arabidopsis Proteins genetics
Mass Spectrometry
Molecular Sequence Data
Mutagenesis, Site-Directed
Phosphopeptides
Phosphorylation
Protein Interaction Mapping
Recombinant Proteins
Sequence Alignment
Transcription Factors genetics
Transcription Factors metabolism
Two-Hybrid System Techniques
Arabidopsis genetics
Arabidopsis Proteins metabolism
Mitogen-Activated Protein Kinase Kinases metabolism
Mitogen-Activated Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 31
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 22134874
- Full Text :
- https://doi.org/10.1007/s00299-011-1192-x