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Ficolin-2 levels and FCN2 haplotypes influence hepatitis B infection outcome in Vietnamese patients.
- Source :
-
PloS one [PLoS One] 2011; Vol. 6 (11), pp. e28113. Date of Electronic Publication: 2011 Nov 22. - Publication Year :
- 2011
-
Abstract
- Human Ficolin-2 (L-ficolins) encoded by FCN2 gene is a soluble serum protein that plays an important role in innate immunity and is mainly expressed in the liver. Ficolin-2 serum levels and FCN2 single nucleotide polymorphisms were associated to several infectious diseases. We initially screened the complete FCN2 gene in 48 healthy individuals of Vietnamese ethnicity. We genotyped a Vietnamese cohort comprising of 423 clinically classified hepatitis B virus patients and 303 controls for functional single nucleotide polymorphisms in the promoter region (-986G>A, -602G>A, -4A>G) and in exon 8 (+6424G>T) by real-time PCR and investigated the contribution of FCN2 genotypes and haplotypes to serum Ficolin-2 levels, viral load and liver enzyme levels. Haplotypes differed significantly between patients and controls (P = 0.002) and the haplotype AGGG was found frequently in controls in comparison to patients with hepatitis B virus and hepatocellular carcinoma (P = 0.0002 and P<0.0001) conferring a protective effect. Ficolin-2 levels differed significantly between patients and controls (p<0.0001). Patients with acute hepatitis B had higher serum Ficolin-2 levels compared to other patient groups and controls.The viral load was observed to be significantly distributed among the haplotypes (P = 0.04) and the AAAG haplotype contributed to higher Ficolin-2 levels and to viral load. Four novel single nucleotide polymorphisms in introns (-941G>T, -310G>A, +2363G>A, +4882G>A) and one synonymous mutation in exon 8 (+6485G>T) was observed. Strong linkage was found between the variant -986G>A and -4A>G. The very first study on Vietnamese cohort associates both Ficolin-2 serum levels and FCN2 haplotypes to hepatitis B virus infection and subsequent disease progression.
- Subjects :
- Adolescent
Adult
Aged
Case-Control Studies
Female
Hepatitis B blood
Humans
Linkage Disequilibrium genetics
Male
Middle Aged
Polymorphism, Single Nucleotide genetics
Vietnam
Viral Load genetics
Young Adult
Ficolins
Genetic Predisposition to Disease
Haplotypes genetics
Hepatitis B genetics
Hepatitis B virology
Hepatitis B virus physiology
Lectins blood
Lectins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 6
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22140517
- Full Text :
- https://doi.org/10.1371/journal.pone.0028113