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In vitro activity of antibiotic combinations against multidrug-resistant strains of Acinetobacter baumannii and the effects of their antibiotic resistance determinants.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2012 Mar; Vol. 328 (1), pp. 26-31. Date of Electronic Publication: 2012 Jan 06. - Publication Year :
- 2012
-
Abstract
- Various combinations of antibiotics are reported to show synergy in treating nosocomial infections with multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii). Here, we studied hospital-acquired outbreak strains of MDR A. baumannii to evaluate optimal combinations of antibiotics. One hundred and twenty-one strains were grouped into one major and one minor clonal group based on repetitive PCR amplification. Twenty representative strains were tested for antibiotic synergy using Etest(®). Five strains were further analyzed by analytical isoelectric focusing and PCR to identify β-lactamase genes or other antibiotic resistance determinants. Our investigation showed that the outbreak strains of MDR A. baumannii belonged to two dominant clones. A combination of colistin and doxycycline showed the best result, being additive or synergistic against 70% of tested strains. Antibiotic additivity was observed more frequently than synergy. Strains possessing the same clonality did not necessarily demonstrate the same response to antibiotic combinations in vitro. We conclude that the effect of antibiotic combinations on our outbreak strains of MDR A. baumannii seemed strain-specific. The bacterial response to antibiotic combinations is probably a result of complex interactions between multiple concomitant antibiotic resistance determinants in each strain.<br /> (© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.)
- Subjects :
- Acinetobacter Infections microbiology
Acinetobacter baumannii classification
Acinetobacter baumannii genetics
Acinetobacter baumannii isolation & purification
Cluster Analysis
Cross Infection microbiology
Drug Interactions
Genotype
Humans
Isoelectric Focusing
Microbial Sensitivity Tests
Molecular Typing
Polymerase Chain Reaction
beta-Lactamases chemistry
beta-Lactamases isolation & purification
Acinetobacter baumannii drug effects
Anti-Bacterial Agents pharmacology
Drug Resistance, Multiple, Bacterial
Subjects
Details
- Language :
- English
- ISSN :
- 1574-6968
- Volume :
- 328
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 22151035
- Full Text :
- https://doi.org/10.1111/j.1574-6968.2011.02480.x