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Leukocyte-associated Ig-like receptor-1-deficient mice have an altered immune cell phenotype.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2012 Jan 15; Vol. 188 (2), pp. 548-58. Date of Electronic Publication: 2011 Dec 07. - Publication Year :
- 2012
-
Abstract
- Cross-linking of the collagen binding receptor leukocyte-associated Ig-like receptor-1 (LAIR-1) in vitro delivers an inhibitory signal that is able to downregulate activation-mediated signals. To study the in vivo function of LAIR-1, we generated LAIR-1(-/-) mice. They are healthy and fertile and have normal longevity; however, they show certain phenotypic characteristics distinct from wild-type mice, including increased numbers of splenic B, regulatory T, and dendritic cells. As LAIR-1(-/-) mice age, the splenic T cell population shows a higher frequency of activated and memory T cells. Because LAIR-1(+/+) and LAIR-1(-/-) T cells traffic with equal proficiency to peripheral lymphoid organs, this is not likely due to abnormal T lymphocyte trafficking. LAIR-1(-/-) mice have lower serum levels of IgG1 and, in response to T-dependent immunization with trinitrophenyl-OVA, switch less efficiently to Ag specific IgG2a and IgG2b, whereas switching to IgG1 is not affected. Several mouse disease models, including experimental autoimmune encephalitis and colitis, were used to examine the effect of LAIR-1 deficiency, and no differences in the responses of LAIR-1(-/-) and LAIR-1(+/+) mice were observed. Taken together, these observations indicate that LAIR-1 plays a role in regulating immune cells and suggest that any adverse effects of its absence may be balanced in vivo by other inhibitory receptors.
- Subjects :
- Animals
B-Lymphocyte Subsets immunology
B-Lymphocyte Subsets metabolism
Dendritic Cells immunology
Dendritic Cells metabolism
Encephalomyelitis, Autoimmune, Experimental genetics
Encephalomyelitis, Autoimmune, Experimental immunology
Encephalomyelitis, Autoimmune, Experimental metabolism
Female
Leukocytes cytology
Leukocytes metabolism
Longevity genetics
Longevity immunology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Receptors, Immunologic physiology
Spleen cytology
Spleen immunology
Spleen metabolism
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets metabolism
T-Lymphocytes, Regulatory immunology
T-Lymphocytes, Regulatory metabolism
Immunophenotyping methods
Leukocytes immunology
Receptors, Immunologic deficiency
Receptors, Immunologic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 188
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 22156345
- Full Text :
- https://doi.org/10.4049/jimmunol.1102044