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Simultaneous determination of diloxanide furoate and metronidazole in presence of diloxanide furoate degradation products.
- Source :
-
Journal of AOAC International [J AOAC Int] 2011 Sep-Oct; Vol. 94 (5), pp. 1427-39. - Publication Year :
- 2011
-
Abstract
- Three methods are presented for the simultaneous determination of diloxanide furoate (DLX) and metronidazole (MTR), used for their antiprotozoal and antiamoebic effect, in the presence of DLX alkaline degradates and in pharmaceutical formulations, without previous separation. The first method is chemometric-assisted spectrophotometry, in which principal component regression and partial least squares were applied. These two approaches were successfully applied to quantify each drug in the mixture using the information included in, the absorption spectra in the range of 225-320 nm. The second method is TLC-densitometry, in which the binary mixture and degradates were separated on silica gel plates using a chloroform-acetone-glacial acetic acid (9.5 + 0.5 + 0.07, v/v/v) mobile phase and the bands were scanned at 254 nm. The last method is HPLC, in which DLX, MTR, and degradates were separated using the mobile phase acetonitrile-0.05 M dibasic potassium phosphate (25 + 75, v/v), adjusted to pH 4 with orthophosphoric acid, at a flow rate of 1 mL/min, on a C18 analytical column. Detection was at 254 nm. The proposed methods were successfully applied for the analysis of DLX and MTR in pharmaceutical formulations, and the results were statistically compared with a reported spectrophotometric method.
- Subjects :
- Calibration
Chemistry, Pharmaceutical
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Densitometry
Drug Combinations
Drug Stability
Drug Storage
Indicators and Reagents
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet
Tablets analysis
Amebicides analysis
Furans analysis
Metronidazole analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1060-3271
- Volume :
- 94
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of AOAC International
- Publication Type :
- Academic Journal
- Accession number :
- 22165007
- Full Text :
- https://doi.org/10.5740/jaoacint.10-286