Back to Search
Start Over
Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.
- Source :
-
PloS one [PLoS One] 2011; Vol. 6 (12), pp. e28921. Date of Electronic Publication: 2011 Dec 09. - Publication Year :
- 2011
-
Abstract
- Background: Odorant binding proteins (OBPs) play important roles in insect olfaction. The brown planthopper (BPH), Nilaparvata lugens Stål (Delphacidae, Auchenorrhyncha, Hemiptera) is one of the most important rice pests. Its monophagy (only feeding on rice), wing form (long and short wing) variation, and annual long distance migration (seeking for rice plants of high nutrition) imply that the olfaction would play a central role in BPH behavior. However, the olfaction related proteins have not been characterized in this insect.<br />Methodology/principal Findings: Full length cDNA of three OBPs were obtained and distinct expression profiles were revealed regarding to tissue, developmental stage, wing form and gender for the first time for the species. The results provide important clues in functional differentiation of these genes. Binding assays with 41 compounds demonstrated that NlugOBP3 had markedly higher binding ability and wider binding spectrum than the other two OBPs. Terpenes and Ketones displayed higher binding while Alkanes showed no binding to the three OBPs. Focused on NlugOBP3, RNA interference experiments showed that NlugOBP3 not only involved in nymph olfaction on rice seedlings, but also had non-olfactory functions, as it was closely related to nymph survival.<br />Conclusions: NlugOBP3 plays important roles in both olfaction and survival of BPH. It may serve as a potential target for developing behavioral disruptant and/or lethal agent in N. lugens.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
DNA, Complementary genetics
Feeding Behavior
Female
Gene Expression Regulation, Developmental
Hemiptera growth & development
Ligands
Male
Molecular Sequence Data
Organ Specificity genetics
Protein Binding
RNA, Messenger genetics
RNA, Messenger metabolism
Receptors, Odorant chemistry
Receptors, Odorant isolation & purification
Recombinant Proteins metabolism
Sequence Alignment
Volatilization
Wings, Animal growth & development
Wings, Animal metabolism
Gene Expression Profiling
Hemiptera genetics
Receptors, Odorant genetics
Receptors, Odorant metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 6
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22174925
- Full Text :
- https://doi.org/10.1371/journal.pone.0028921