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Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS.
- Source :
-
Bioanalysis [Bioanalysis] 2011 Dec; Vol. 3 (24), pp. 2783-96. - Publication Year :
- 2011
-
Abstract
- Background: Low-dose methotrexate is used for the treatment of rheumatoid arthritis and juvenile idiopathic arthritis, but its effectiveness greatly varies between individuals. Therapeutic drug monitoring of intracellular methotrexate metabolites, the γ-polyglutamates (MTXGlu(n)), in human erythrocytes has shown promise in providing a basis for individualization of therapy.<br />Results: This work presents expedient methodology for the analysis of MTXGlu(1-7) in human erythrocytes by ion-pair UPLC with detection by tandem MS (UPLC-ESI-MS/MS). The use of N,N-dimethylheptylamine as an ion-pair agent was found to be favorable over others. Thermal extraction of erythrocyte lysates provides a simple one-step extraction procedure. The entire chromatographic run time is 6 min and the assay was validated within the therapeutic range of these metabolites<br />Conclusion: The developed sample preparation procedure in combination with ion-pair UPLC-ESI-MS/MS analysis allowed for expedient quantitation of MTXGlu(1-7) in human erythrocytes. The rapid analysis time would enable therapeutic drug monitoring of MTXGlu(1-7) in the clinic.
Details
- Language :
- English
- ISSN :
- 1757-6199
- Volume :
- 3
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- Bioanalysis
- Publication Type :
- Academic Journal
- Accession number :
- 22185278
- Full Text :
- https://doi.org/10.4155/bio.11.288