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Iberian wolf as a reservoir of extended-spectrum β-lactamase-producing Escherichia coli of the TEM, SHV, and CTX-M groups.
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2012 Apr; Vol. 18 (2), pp. 215-9. Date of Electronic Publication: 2011 Dec 20. - Publication Year :
- 2012
-
Abstract
- The intensive use of antibiotics in human and veterinary medicine, associated with mechanisms of bacterial genetic transfer, caused a selective pressure that contributed to the dissemination of antimicrobial resistance in different bacteria groups and throughout different ecosystems. Iberian wolf, due to his predatory and wild nature, may serve as an important indicator of environmental contamination with antimicrobial resistant bacteria. The aim of this study was to characterize the diversity of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates within the fecal microbiota of Iberian wolf. Additionally, the identification of other associated resistance genes, phylogenetic groups, and the detection of virulence determinants were also focused on in this study. From 2008 to 2009, 237 fecal samples from Iberian wolf were collected in Portugal. E. coli isolates with TEM-52, SHV-12, CTX-M-1, and CTX-M-14-type ESBLs were detected in 13 of these samples (5.5%). This study reveals the presence of ESBL-producing E. coli isolates, in a wild ecosystem, which could be disseminated through the environment. Moreover, the presence of resistant genes in integrons and the existence of virulence determinants were shown. The association between antibiotic resistance and virulence determinants should be monitored, as it constitutes a serious public health problem.
- Subjects :
- Animals
DNA, Bacterial genetics
Drug Resistance, Bacterial genetics
Electrophoresis, Gel, Pulsed-Field
Escherichia coli genetics
Escherichia coli isolation & purification
Escherichia coli Infections microbiology
Escherichia coli Proteins genetics
Escherichia coli Proteins metabolism
Feces microbiology
Integrons
Microbial Sensitivity Tests
Phylogeny
Portugal
Virulence Factors genetics
beta-Lactamases classification
beta-Lactamases genetics
Anti-Bacterial Agents pharmacology
Escherichia coli drug effects
Escherichia coli enzymology
Escherichia coli Infections veterinary
Wolves microbiology
beta-Lactamases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 18
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 22185366
- Full Text :
- https://doi.org/10.1089/mdr.2011.0145