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Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.
- Source :
-
PLoS pathogens [PLoS Pathog] 2011 Dec; Vol. 7 (12), pp. e1002438. Date of Electronic Publication: 2011 Dec 15. - Publication Year :
- 2011
-
Abstract
- Host factors are recruited into viral replicase complexes to aid replication of plus-strand RNA viruses. In this paper, we show that deletion of eukaryotic translation elongation factor 1Bgamma (eEF1Bγ) reduces Tomato bushy stunt virus (TBSV) replication in yeast host. Also, knock down of eEF1Bγ level in plant host decreases TBSV accumulation. eEF1Bγ binds to the viral RNA and is one of the resident host proteins in the tombusvirus replicase complex. Additional in vitro assays with whole cell extracts prepared from yeast strains lacking eEF1Bγ demonstrated its role in minus-strand synthesis by opening of the structured 3' end of the viral RNA and reducing the possibility of re-utilization of (+)-strand templates for repeated (-)-strand synthesis within the replicase. We also show that eEF1Bγ plays a synergistic role with eukaryotic translation elongation factor 1A in tombusvirus replication, possibly via stimulation of the proper positioning of the viral RNA-dependent RNA polymerase over the promoter region in the viral RNA template.These roles for translation factors during TBSV replication are separate from their canonical roles in host and viral protein translation.
- Subjects :
- Gene Knockdown Techniques
Mutagenesis genetics
Mutation genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae virology
Saccharomyces cerevisiae Proteins metabolism
Tombusvirus genetics
Virus Replication physiology
Peptide Elongation Factor 1 metabolism
RNA, Viral biosynthesis
RNA-Dependent RNA Polymerase metabolism
Tombusvirus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7374
- Volume :
- 7
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- PLoS pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 22194687
- Full Text :
- https://doi.org/10.1371/journal.ppat.1002438