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Application of IGF2-specific identifier probe for cytogenetic study of somatic and sperm cells in horses.

Application of IGF2-specific identifier probe for cytogenetic study of somatic and sperm cells in horses.

Authors :
Potocki L
Bugno-Poniewierska M
Tischner M
Wnuk M
Source :
Folia biologica [Folia Biol (Krakow)] 2011; Vol. 59 (3-4), pp. 147-9.
Publication Year :
2011

Abstract

The development of molecular techniques with fluorochromes has had an invaluable impact on discovering the nature of chromatin structure. Here, we show the application of a locus specific identifier probe (LSI) for precise and selective visualization of the horse IGF2 gene in the metaphase, interphase nuclei and sperm cells. Our study may be helpful for interpretation of results of interphase fluorescence in situ hybridization (I-FISH). We analyze and discuss the variation in the number and localization of FISH signals in somatic and sperm cells of horse.

Details

Language :
English
ISSN :
0015-5497
Volume :
59
Issue :
3-4
Database :
MEDLINE
Journal :
Folia biologica
Publication Type :
Academic Journal
Accession number :
22195468
Full Text :
https://doi.org/10.3409/fb59_3-4.147-149