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The differential modulation of USP activity by internal regulatory domains, interactors and eight ubiquitin chain types.
- Source :
-
Chemistry & biology [Chem Biol] 2011 Dec 23; Vol. 18 (12), pp. 1550-61. - Publication Year :
- 2011
-
Abstract
- Ubiquitin-specific proteases (USPs) are papain-like isopeptidases with variable inter- and intramolecular regulatory domains. To understand the effect of these domains on USP activity, we have analyzed the enzyme kinetics of 12 USPs in the presence and absence of modulators using synthetic reagents. This revealed variations of several orders of magnitude in both the catalytic turnover (k(cat)) and ubiquitin (Ub) binding (K(M)) between USPs. Further activity modulation by intramolecular domains affects both the k(cat) and K(M), whereas the intermolecular activators UAF1 and GMPS mainly increase the k(cat). Also, we provide the first comprehensive analysis comparing Ub chain preference. USPs can hydrolyze all linkages and show modest Ub-chain preferences, although some show a lack of activity toward linear di-Ub. This comprehensive kinetic analysis highlights the variability within the USP family.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Catalytic Domain
Endopeptidases chemistry
Endopeptidases genetics
Guanosine Monophosphate metabolism
Humans
Kinetics
Nuclear Proteins metabolism
Protein Binding
Protein Structure, Tertiary
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Thionucleotides metabolism
Ubiquitin chemistry
Ubiquitin-Specific Proteases
Endopeptidases metabolism
Ubiquitin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1301
- Volume :
- 18
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Chemistry & biology
- Publication Type :
- Academic Journal
- Accession number :
- 22195557
- Full Text :
- https://doi.org/10.1016/j.chembiol.2011.10.017