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Anti-Sp17 monoclonal antibody with antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity activities against human ovarian cancer cells.
- Source :
-
Medical oncology (Northwood, London, England) [Med Oncol] 2012 Dec; Vol. 29 (4), pp. 2923-31. Date of Electronic Publication: 2011 Dec 24. - Publication Year :
- 2012
-
Abstract
- Sperm protein 17 (Sp17) is a cancer testis antigen that has been shown to be overexpressed in a variety of gynecologic malignancies, in particular ovarian cancer. Emerging evidences indicate that Sp17 is involved in tumorigenesis and in the migration of malignant cells. It has been proposed as a useful target for tumor-vaccine strategies and a novel marker to define tumor subsets and predict drug response. However, the antitumor activity of anti-Sp17 monoclonal antibody (anti-Sp17 mAb) has not been investigated. In this study, the in vitro cytotoxicity, antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) activities of anti-Sp17 mAb were evaluated using Sp17-positive ovarian cancer cells as targets, Sp17-negative ovarian cancer cells as the control, and healthy human peripheral blood monocytes and healthy human serum as effectors. Our preliminary results indicate that the direct cytotoxicity of anti-Sp17 mAb against the investigated ovarian cancer cells was very weak. However, the cytotoxicity of anti-Sp17 mAb, mediated by peripheral blood mononuclear cells (PBMCs), as ADCC, or by human serum, as CDC, was relatively strong in the Sp17-positive ovarian cancer cells. This finding suggested that anti-Sp17 mAb could be a useful tool against ovarian cancer and may provide insight into the development of low side-effect targeting therapy for this malignant disease.
- Subjects :
- Calmodulin-Binding Proteins
Cell Line, Tumor
Female
Humans
Membrane Proteins
Ovarian Neoplasms immunology
Antibodies, Monoclonal therapeutic use
Antibody-Dependent Cell Cytotoxicity
Antigens, Surface immunology
Carrier Proteins immunology
Complement System Proteins immunology
Cytotoxicity, Immunologic
Ovarian Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1559-131X
- Volume :
- 29
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Medical oncology (Northwood, London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 22198696
- Full Text :
- https://doi.org/10.1007/s12032-011-0137-0