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Pathogenic and genetic diversity of Xanthomonas translucens pv. undulosa in North Dakota.
- Source :
-
Phytopathology [Phytopathology] 2012 Apr; Vol. 102 (4), pp. 390-402. - Publication Year :
- 2012
-
Abstract
- Bacterial leaf streak (BLS), caused by Xanthomonas translucens pv. undulosa, has become more prevalent recently in North Dakota and neighboring states. From five locations in North Dakota, 226 strains of X. translucens pv. undulosa were collected and evaluated for pathogenicity and then selected strains were inoculated on a set of 12 wheat cultivars and other cereal hosts. The genetic diversity of all strains was determined using repetitive sequence-based polymerase chain reaction (rep-PCR) and insertion sequence-based (IS)-PCR. Bacterial strains were pathogenic on wheat and barley but symptom severity was greatest on wheat. Strains varied greatly in aggressiveness, and wheat cultivars also showed differential responses to several strains. The 16S ribosomal DNA sequences of the strains were identical, and distinct from those of the other Xanthomonas pathovars. Combined rep-PCR and IS-PCR data produced 213 haplotypes. Similar haplotypes were detected in more than one location. Although diversity was greatest (≈92%) among individuals within a location, statistically significant (P ≤ 0.001 or 0.05) genetic differentiation among locations was estimated, indicating geographic differentiation between pathogen populations. The results of this study provide information on the pathogen diversity in North Dakota, which will be useful to better identify and characterize resistant germplasm.
- Subjects :
- Base Sequence
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Genetics, Population
Geography
Haplotypes
Linkage Disequilibrium
Molecular Sequence Data
North Dakota
Phenotype
RNA, Ribosomal, 16S genetics
Repetitive Sequences, Nucleic Acid genetics
Sequence Analysis, DNA
Virulence
Xanthomonas isolation & purification
Xanthomonas physiology
Genetic Variation genetics
Plant Diseases microbiology
Triticum microbiology
Xanthomonas genetics
Xanthomonas pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 0031-949X
- Volume :
- 102
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Phytopathology
- Publication Type :
- Academic Journal
- Accession number :
- 22204654
- Full Text :
- https://doi.org/10.1094/PHYTO-07-11-0201