Back to Search
Start Over
The use of magnetic resonance cell tracking to monitor endothelial progenitor cells in a rat hindlimb ischemic model.
- Source :
-
Biomaterials [Biomaterials] 2012 Mar; Vol. 33 (8), pp. 2439-48. Date of Electronic Publication: 2011 Dec 27. - Publication Year :
- 2012
-
Abstract
- A water-soluble magnetic resonance imaging (MRI) contrast agent, Dextran mono-N-succinimidyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate-gadolinium(3+) (Dex-DOTA-Gd(3+)), was shown to enable monitoring of the anatomical migration and the survival period of transplanted stem cells for up to 1 month. Gadolinium molecules in the cells were rapidly eliminated from the site and excreted upon cell death. Endothelial progenitor cells (EPCs) transplanted into the inguinal femoral muscle of rats migrated distally through the knee in rats after hindlimb ischemia but did not migrate in non-ischemic rats. Interestingly, the survival period of transplanted EPCs was notably prolonged in the ischemic limb, indicating that EPCs are required by the ischemic tissues and that the fate of transplanted EPCs was affected by the disease. Compared to the commonly used particle type of MRI contrast agents, the system described in this study is expected to be invaluable to help clarifying the process of stem cell transplantation therapy.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cell Movement drug effects
Dextrans chemistry
Dextrans pharmacology
Disease Models, Animal
Endothelial Cells drug effects
Endothelial Cells metabolism
Gadolinium pharmacokinetics
Heterocyclic Compounds chemistry
Heterocyclic Compounds pharmacology
Hindlimb drug effects
Hindlimb pathology
Hindlimb physiopathology
Imaging, Three-Dimensional
Ischemia pathology
Ischemia physiopathology
Male
Organometallic Compounds chemistry
Organometallic Compounds pharmacology
Rats
Rats, Inbred F344
Regional Blood Flow drug effects
Stem Cells drug effects
Stem Cells metabolism
Wound Healing drug effects
Cell Tracking methods
Endothelial Cells cytology
Hindlimb blood supply
Ischemia therapy
Magnetic Resonance Spectroscopy methods
Stem Cell Transplantation
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 33
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 22206594
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2011.11.075