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In vivo monitoring of venous thrombosis in mice.
- Source :
-
Journal of thrombosis and haemostasis : JTH [J Thromb Haemost] 2012 Mar; Vol. 10 (3), pp. 447-52. - Publication Year :
- 2012
-
Abstract
- Background: Venous thrombosis (VT) is an important cause of morbidity and mortality in clinical medicine. Animal models studying venous thrombosis are scarce and, in most cases, very crude and rely on sacrificing the animals to excise formed thrombi. Developing an in vivo murine model of venous thrombosis can be a powerful tool for studying venous thrombosis.<br />Objectives: We sought to use a high-frequency ultrasound system (HFUS) to dynamically and non-invasively monitor thrombus formation in the inferior vena cava (IVC) of mice.<br />Methods: We developed a murine model of venous thrombosis using, for detection, the Vevo 770(®), a micro-imaging HFUS. Two different thrombosis models were used to generate thrombi in the IVC of C57Bl/6NCr mice: (i) ligation and (ii) application of ferric chloride (FeCl(3)). We then assessed venous thrombosis by HFUS.<br />Results: In both models, measurements of the clot pathologically correlated favorably with measurements acquired with HFUS. Thrombus develops less than an hour after ligation or FeCl(3) -induced injury of the IVC and the size of the clot increases over time for up to 24 h. Importantly, we demonstrate that HFUS can be used to monitor the effect of an anticoagulant such as dalteparin until complete resolution of the thrombus.<br />Conclusions: These data show that HFUS assesses venous thrombosis in mice reliably and non-invasively. Developing a murine model of thrombosis using more accurate, and clinically more relevant, techniques such as ultrasonography, is a step towards a better understanding of the pathophysiology of venous thromboembolism.<br /> (© 2011 International Society on Thrombosis and Haemostasis.)
- Subjects :
- Animals
Anticoagulants pharmacology
Chlorides
Dalteparin pharmacology
Disease Models, Animal
Ferric Compounds
Ligation
Male
Mice
Mice, Inbred C57BL
Time Factors
Vena Cava, Inferior surgery
Venous Thrombosis blood
Venous Thrombosis drug therapy
Venous Thrombosis etiology
Blood Coagulation drug effects
Monitoring, Physiologic methods
Ultrasonography, Doppler, Color
Ultrasonography, Doppler, Pulsed
Vena Cava, Inferior diagnostic imaging
Venous Thrombosis diagnostic imaging
Subjects
Details
- Language :
- English
- ISSN :
- 1538-7836
- Volume :
- 10
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of thrombosis and haemostasis : JTH
- Publication Type :
- Academic Journal
- Accession number :
- 22212403
- Full Text :
- https://doi.org/10.1111/j.1538-7836.2011.04615.x