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Disinfection of flexible fiberoptic laryngoscopes after in vitro contamination with Staphylococcus aureus and Candida albicans.

Authors :
Chang D
Florea A
Rowe M
Seiberling KA
Source :
Archives of otolaryngology--head & neck surgery [Arch Otolaryngol Head Neck Surg] 2012 Feb; Vol. 138 (2), pp. 119-21. Date of Electronic Publication: 2012 Jan 16.
Publication Year :
2012

Abstract

Objective: To determine the efficacy of various cleaning and disinfective methods in reducing bacterial and fungal load on flexible fiberoptic laryngoscopes (FFLs).<br />Design: In vitro model.<br />Subjects: Flexible fiberoptic laryngoscopes contaminated with Staphylococcus aureus and Candida albicans.<br />Interventions: Contamination with S aureus and C albicans was separately induced on FFLs, which were then disinfected with different protocols: 20-, 15-, 10-, and 5-minute soaks in ortho-phthalaldehyde (Cidex OPA; Johnson & Johnson) with or without presoaking in an enzymatic soap solution for 5 minutes; an isolated 5-minute soak in an enzymatic soap solution; a 30-second wipe with antibacterial soap and water; a 30-second wipe with isopropyl alcohol; a 30-second wipe with antibacterial soap, followed by a 30-second scrub with isopropyl alcohol; and a 30-second wipe with germicidal cloth, all accompanied by previous rinsing with 30 seconds of running tap water.<br />Results: All protocols except the isolated 5-minute soak in enzymatic soap solution were successful in completely disinfecting the FFLs after experimental contamination with S aureus or C albicans.<br />Conclusion: Various different cleaning methods appeared to properly disinfect FFLs after inoculation with S aureus and C albicans in an in vitro model.

Details

Language :
English
ISSN :
1538-361X
Volume :
138
Issue :
2
Database :
MEDLINE
Journal :
Archives of otolaryngology--head & neck surgery
Publication Type :
Academic Journal
Accession number :
22248562
Full Text :
https://doi.org/10.1001/archoto.2011.1204