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Protocadherin8 is a functional tumor suppressor frequently inactivated by promoter methylation in nasopharyngeal carcinoma.
- Source :
-
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) [Eur J Cancer Prev] 2012 Nov; Vol. 21 (6), pp. 569-75. - Publication Year :
- 2012
-
Abstract
- Protocadherin8 (PCDH8), an integral membrane protein, was reported to be a tumor suppressor involved in tumorigenesis in cancers. We aimed to investigate the epigenetic inactivation of PCDH8 and its tumor-suppressor function in nasopharyngeal carcinoma (NPC). Frequent downregulation/silencing of PCDH8 was found in NPC cell lines using semiquantitative PCR. Promoter methylation of PCDH8 was observed in 100% (5/5) of cell lines and 85.3% (35/41) of primary tumors by methylation-specific PCR, but not in normal nasopharyngeal tissues. Treatment of NPC cells with 5-aza-2'-deoxycytidine and/or trichostatin A could restore PCDH8 expression. Ectopic expression of PCDH8 in silenced NPC cells significantly inhibited cell colony formation and cell migration. For the first time, our study demonstrates the epigenetic inactivation of PCDH8 by promoter methylation and its tumor-suppressive function in NPC. Thus, PCDH8 could be identified as a tumor suppressor in NPC.
- Subjects :
- Apoptosis drug effects
Azacitidine analogs & derivatives
Azacitidine pharmacology
Cell Adhesion drug effects
Cell Movement drug effects
Cell Proliferation drug effects
Decitabine
Female
Humans
Hydroxamic Acids pharmacology
Male
Middle Aged
Nasopharyngeal Neoplasms drug therapy
Nasopharyngeal Neoplasms pathology
Prognosis
Protocadherins
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Wound Healing
Cadherins genetics
DNA Methylation
Gene Expression Regulation, Neoplastic
Nasopharyngeal Neoplasms genetics
Promoter Regions, Genetic genetics
Tumor Suppressor Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1473-5709
- Volume :
- 21
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)
- Publication Type :
- Academic Journal
- Accession number :
- 22273848
- Full Text :
- https://doi.org/10.1097/CEJ.0b013e328350b097