Back to Search
Start Over
[Analyses of IDH1 mutation and MGMT promoter methylation status for 5 cases of long-term survivors with glioblastoma].
- Source :
-
No shinkei geka. Neurological surgery [No Shinkei Geka] 2012 Feb; Vol. 40 (2), pp. 129-35. - Publication Year :
- 2012
-
Abstract
- Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with an extremely poor prognosis in spite of multimodal treatment approaches. The median survival time of patients with GBM is 15 months, and only 3-5% of patients survive longer than 36 months. Those patients who survive over 36 months after the initial diagnosis are defined as long-term survivors. In this study, we retrospectively performed clinical and molecular analyses of five long-term survivors of GBM (>36 months) and twenty four GBM patients with poor survival time as control group (<36 months) to identify any prognostic factors that potentially contribute to survival. The O <superscript>6</superscript> -methylguanine-DNA methyltransferase (MGMT) gene methylation status was evaluated by performing methylation specific polymerase chain reaction assays. The mutation of isocitrate dehydrogenase 1 and 2 were evaluated by the direct sequencing method. All five cases were primary GBMs and the coexistence of the oligodendroglioma component was checked for each case as GBM with oligodendroglioma component. All five cases showed MGMT promoter methylation (5/5). IDH1 mutation was detected in two of the long-term survivors with oligodendroglioma component (2/5) while no IDH1 mutation was detected in the control group. All patients were treated by gross total removal followed by radiotherapy and various chemotherapies including temozolomide. MGMT promoter methylation and IDH1 mutation might be favorable factors for long-term survival in GBM patients.
- Subjects :
- Adult
Aged
Biomarkers analysis
Female
Humans
Male
Middle Aged
Prognosis
Retrospective Studies
Survivors
Brain Neoplasms mortality
DNA Methylation
DNA Modification Methylases genetics
DNA Repair Enzymes genetics
Glioblastoma mortality
Isocitrate Dehydrogenase genetics
Mutation
Promoter Regions, Genetic physiology
Tumor Suppressor Proteins genetics
Subjects
Details
- Language :
- Japanese
- ISSN :
- 0301-2603
- Volume :
- 40
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- No shinkei geka. Neurological surgery
- Publication Type :
- Academic Journal
- Accession number :
- 22281465