Back to Search
Start Over
Resveratrol reduces vascular cell senescence through attenuation of oxidative stress by SIRT1/NADPH oxidase-dependent mechanisms.
- Source :
-
The Journal of nutritional biochemistry [J Nutr Biochem] 2012 Nov; Vol. 23 (11), pp. 1410-6. Date of Electronic Publication: 2012 Jan 27. - Publication Year :
- 2012
-
Abstract
- Objective: Senescence of vascular cells contributes to the development of cardiovascular diseases and the overall aging. This study was undertaken to investigate the effects of resveratrol (Res) on amelioration of vascular cell aging and the role of SIRT1/nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathway.<br />Methods and Results: Adult male Wistar rats were treated with a high-fat/sucrose diet (HFS) in the presence or absence of Res for 3 months. HFS and in vitro treatment with high glucose increased the senescence cells and reactive oxygen species production in rat aorta and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Res protected against HFS- or high-glucose-induced increase in NADPH oxidase p47phox expression and decrease in SIRT1 level. Apocynin, a NADPH oxidase inhibitor, down-regulated p47phox protein expression, but had no influence on SIRT1 protein; sirtinol, a SIRT1 inhibitor, aggravated the decrease in SIRT1 protein level and the increase in p47phox protein expression induced by high glucose.<br />Conclusion: Our studies suggested that Res was able to reverse the senescence process in aorta induced by HFS in rats or induced by the exposure to high glucose in cultured BAECs. The underlying mechanism is at least SIRT1/NADPH oxidase pathway dependent.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Aorta cytology
Aorta drug effects
Body Weight drug effects
Cattle
Cells, Cultured
Diet, High-Fat adverse effects
Endothelium, Vascular drug effects
Glucose adverse effects
Lipids blood
Male
Rats
Rats, Wistar
Reactive Oxygen Species metabolism
Resveratrol
Cellular Senescence drug effects
Endothelium, Vascular cytology
NADPH Oxidases metabolism
Oxidative Stress drug effects
Sirtuin 1 metabolism
Stilbenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4847
- Volume :
- 23
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of nutritional biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22284404
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2011.08.008