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siRNA-mediated knockdown of FoxP3 promotes the ratio of T-helper 1 (Th1) to Th2 in chronic hepatitis B patients.
- Source :
-
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology [Turk J Gastroenterol] 2011 Dec; Vol. 22 (6), pp. 587-93. - Publication Year :
- 2011
-
Abstract
- To determine the effect of regulatory T cells on the ratio of Th1/Th2 differentiation in peripheral blood mononuclear cells of chronic hepatitis B patients, FoxP3, the essential transcription factor for Tcells differentiation and function, was knocked down by FoxP3 siRNA, which was affirmed by real-time polymerase chain reaction for decreased expression of FoxP3. Then, at different time points, comparing with control groups, we detected enhanced lymphocyte proliferation and up-regulated Th1-type cytokines, but down-regulated Th2-type cytokines by ELISA. Finally, to research the involved mechanisms, an increased ratio of T-bet/GATA-3 mRNA expression was found by real-time polymerase chain reaction. Our results suggest that siRNA-mediated knockdown of FoxP3 could regulate the immune balance of Th1/Th2 in chronic hepatitis B patients, which may be mediated partly by regulating transcription factors T-bet and GATA-3.
- Subjects :
- Adult
Cell Proliferation
Cells, Cultured
Down-Regulation
Female
GATA3 Transcription Factor genetics
Gene Expression Regulation
Hepatitis B, Chronic genetics
Humans
Interferon-gamma metabolism
Interleukin-10 metabolism
Interleukin-2 metabolism
Interleukin-4 metabolism
Lymphocyte Count
Male
Middle Aged
RNA, Small Interfering
T-Box Domain Proteins genetics
Up-Regulation
Forkhead Transcription Factors genetics
Hepatitis B, Chronic blood
RNA, Messenger metabolism
T-Lymphocytes, Regulatory metabolism
Th1 Cells metabolism
Th2 Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2148-5607
- Volume :
- 22
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 22287403
- Full Text :
- https://doi.org/10.4318/tjg.2011.0251