Back to Search Start Over

Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs.

Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs.

Authors :
Davis ME
Lisowyj MP
Zhou L
Wisecarver JL
Gulizia JM
Shostrom VK
Naud N
Corpet DE
Mirvish SS
Source :
Nutrition and cancer [Nutr Cancer] 2012; Vol. 64 (2), pp. 342-9. Date of Electronic Publication: 2012 Jan 31.
Publication Year :
2012

Abstract

Nitrite-preserved meats (e.g., hot dogs) may help cause colon cancer because they contain N-nitroso compounds. We tested whether purified hot-dog-derived total apparent N-nitroso compounds (ANC) could induce colonic aberrant crypts, which are putative precursors of colon cancer. We purified ANC precursors in hot dogs and nitrosated them to produce ANC. In preliminary tests, CF1 mice received 1 or 3 i.p. injections of 5 mg azoxymethane (AOM)/kg. In Experiments 1 and 2, female A/J mice received ANC in diet. In Experiment 1, ANC dose initially dropped sharply because the ANC precursors had mostly decomposed but, later in Experiment 1 and throughout Experiment 2, ANC remained at 85 nmol/g diet. Mice were killed after 8 (AOM tests) or 17-34 (ANC tests) wk. Median numbers of aberrant crypts in the distal 2 cm of the colon for 1 and 3 AOM injections, CF1 controls, ANC (Experiment 1), ANC (Experiment 2),and untreated A/J mice were 31, 74, 12, 20, 12, and 5-6, with P < 0.01 for both ANC tests. Experiment 2 showed somewhat increased numbers of colonic mucin-depleted foci in the ANC-treated group. We conclude that hot-dog-derived ANC induced significant numbers of aberrant crypts in the mouse colon.

Details

Language :
English
ISSN :
1532-7914
Volume :
64
Issue :
2
Database :
MEDLINE
Journal :
Nutrition and cancer
Publication Type :
Academic Journal
Accession number :
22293095
Full Text :
https://doi.org/10.1080/01635581.2012.650777