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Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs.
Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs.
- Source :
-
Nutrition and cancer [Nutr Cancer] 2012; Vol. 64 (2), pp. 342-9. Date of Electronic Publication: 2012 Jan 31. - Publication Year :
- 2012
-
Abstract
- Nitrite-preserved meats (e.g., hot dogs) may help cause colon cancer because they contain N-nitroso compounds. We tested whether purified hot-dog-derived total apparent N-nitroso compounds (ANC) could induce colonic aberrant crypts, which are putative precursors of colon cancer. We purified ANC precursors in hot dogs and nitrosated them to produce ANC. In preliminary tests, CF1 mice received 1 or 3 i.p. injections of 5 mg azoxymethane (AOM)/kg. In Experiments 1 and 2, female A/J mice received ANC in diet. In Experiment 1, ANC dose initially dropped sharply because the ANC precursors had mostly decomposed but, later in Experiment 1 and throughout Experiment 2, ANC remained at 85 nmol/g diet. Mice were killed after 8 (AOM tests) or 17-34 (ANC tests) wk. Median numbers of aberrant crypts in the distal 2 cm of the colon for 1 and 3 AOM injections, CF1 controls, ANC (Experiment 1), ANC (Experiment 2),and untreated A/J mice were 31, 74, 12, 20, 12, and 5-6, with P < 0.01 for both ANC tests. Experiment 2 showed somewhat increased numbers of colonic mucin-depleted foci in the ANC-treated group. We conclude that hot-dog-derived ANC induced significant numbers of aberrant crypts in the mouse colon.
- Subjects :
- Animals
Azoxymethane administration & dosage
Azoxymethane toxicity
Feces chemistry
Female
Food Handling
Meat Products analysis
Mice
Nitrosation
Nitroso Compounds analysis
Sodium Nitrite administration & dosage
Sodium Nitrite metabolism
Aberrant Crypt Foci chemically induced
Carcinogens toxicity
Colonic Neoplasms chemically induced
Meat Products toxicity
Nitroso Compounds toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1532-7914
- Volume :
- 64
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nutrition and cancer
- Publication Type :
- Academic Journal
- Accession number :
- 22293095
- Full Text :
- https://doi.org/10.1080/01635581.2012.650777