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In-cell solid-state NMR as a tool to study proteins in large complexes.
- Source :
-
Chembiochem : a European journal of chemical biology [Chembiochem] 2012 Mar 05; Vol. 13 (4), pp. 534-7. Date of Electronic Publication: 2012 Feb 01. - Publication Year :
- 2012
-
Abstract
- A major limitation of solution NMR is molecular tumbling, which is often too slow for detection. Here we demonstrate that solid-state NMR spectroscopy in combination with flash freezing of cells can be used to detect proteins in the cellular environment and provides information on backbone chemical shifts.<br /> (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Details
- Language :
- English
- ISSN :
- 1439-7633
- Volume :
- 13
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Chembiochem : a European journal of chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 22298299
- Full Text :
- https://doi.org/10.1002/cbic.201100721