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Dual regulation by ethanol of the inhibitory effects of ketamine on spinal NMDA-induced pressor responses in rats.
Dual regulation by ethanol of the inhibitory effects of ketamine on spinal NMDA-induced pressor responses in rats.
- Source :
-
Journal of biomedical science [J Biomed Sci] 2012 Feb 02; Vol. 19, pp. 11. Date of Electronic Publication: 2012 Feb 02. - Publication Year :
- 2012
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Abstract
- Background: Acute exposure of ethanol (alcohol) inhibits NMDA receptor function. Our previous study showed that acute ethanol inhibited the pressor responses induced by NMDA applied intrathecally; however, prolonged ethanol exposure may increase the levels of phosphorylated NMDA receptor subunits leading to changes in ethanol inhibitory potency on NMDA-induced responses. The present study was carried out to examine whether acute ethanol exposure influences the effects of ketamine, a noncompetitive NMDA receptor antagonist, on spinal NMDA-induced pressor responses.<br />Methods: The blood pressure responses induced by intrathecal injection of NMDA were recorded in urethane-anesthetized rats weighing 250-275 g. The levels of several phosphorylated residues on NMDA receptor GluN1 subunits were determined by western blot analysis.<br />Results: Intravenous injection of ethanol or ketamine inhibited spinal NMDA-induced pressor responses in a dose-dependent and reversible manner. Ketamine inhibition of NMDA-induced responses was synergistically potentiated by ethanol when ethanol was applied just before ketamine. However, ketamine inhibition was significantly reduced when applied at 10 min after ethanol administration. Western blot analysis showed that intravenous ethanol increased the levels of phosphoserine 897 on GluN1 subunits (pGluN1-serine 897), selectively phosphorylated by protein kinase A (PKA), in the lateral horn regions of spinal cord at 10 min after administration. Intrathecal administration of cAMPS-Sp, a PKA activator, at doses elevating the levels of pGluN1-serine 897, significantly blocked ketamine inhibition of spinal NMDA-induced responses.<br />Conclusions: The results suggest that ethanol may differentially regulate ketamine inhibition of spinal NMDA receptor function depending on ethanol exposure time and the resulting changes in the levels of pGluN1-serine 897.
- Subjects :
- Animals
Blotting, Western
Cyclic AMP administration & dosage
Cyclic AMP pharmacology
Cyclic AMP-Dependent Protein Kinases antagonists & inhibitors
Injections, Spinal
Ketamine antagonists & inhibitors
Male
N-Methylaspartate administration & dosage
Phosphorylation
Phosphoserine metabolism
Rats
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate agonists
Receptors, N-Methyl-D-Aspartate antagonists & inhibitors
Spinal Cord drug effects
Thionucleotides administration & dosage
Time Factors
Cyclic AMP analogs & derivatives
Cyclic AMP-Dependent Protein Kinases physiology
Ethanol toxicity
Ketamine pharmacology
N-Methylaspartate pharmacology
Receptors, N-Methyl-D-Aspartate metabolism
Spinal Cord metabolism
Thionucleotides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0127
- Volume :
- 19
- Database :
- MEDLINE
- Journal :
- Journal of biomedical science
- Publication Type :
- Academic Journal
- Accession number :
- 22300389
- Full Text :
- https://doi.org/10.1186/1423-0127-19-11