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Dermacentor variabilis: characterization and modeling of macrophage migration inhibitory factor with phylogenetic comparisons to other ticks, insects and parasitic nematodes.
- Source :
-
Experimental parasitology [Exp Parasitol] 2012 Mar; Vol. 130 (3), pp. 232-8. Date of Electronic Publication: 2012 Jan 24. - Publication Year :
- 2012
-
Abstract
- We have identified and characterized the full length cDNA sequence of macrophage migration inhibitory factor (MIF) from the American dog tick, Dermacentor variabilis. The nucleotide and putative amino acid sequences from this study shared a high level of sequence conservation with other tick MIFs. The bioinformatics analysis showed across species conservation of the MIF amino acid sequence in ticks, insects and nematodes. The multiple sequence alignment identified Pro 1, 3, 55; Thr 7, 112; Asn 8, 72; Ile 64, 96; Gly 65, 110, Ser 63 and Leu 87 amino acids to be highly conserved among the sequences selected for this study. Tick MIF does not have the oxidoreductase domain as found in MIFs from other animals suggesting that tick MIF is not capable of performing as an oxidoreductase. The phylogenetic analysis revealed that tick MIFs share a closer evolutionary proximity to parasitic nematode MIFs than to insect MIFs.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Arachnid Vectors classification
Arachnid Vectors genetics
Base Sequence
Conserved Sequence
DNA, Complementary chemistry
Dermacentor classification
Dermacentor genetics
Female
Insecta classification
Insecta genetics
Macrophage Migration-Inhibitory Factors chemistry
Models, Molecular
Molecular Sequence Data
Nematoda classification
Nematoda genetics
Phylogeny
Rabbits
Sequence Alignment
Sequence Homology, Amino Acid
Sheep
Ticks classification
Ticks genetics
Ticks metabolism
Arachnid Vectors metabolism
Dermacentor metabolism
Insecta metabolism
Macrophage Migration-Inhibitory Factors genetics
Nematoda metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 130
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 22306068
- Full Text :
- https://doi.org/10.1016/j.exppara.2011.12.010