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Ribozyme-mediated trans insertion-splicing into target RNAs.

Authors :
Dotson PP 2nd
Hart J
Noe C
Testa SM
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 848, pp. 385-94.
Publication Year :
2012

Abstract

The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.

Subjects

Subjects :
Binding Sites
Oligoribonucleotides genetics
Oligoribonucleotides metabolism
Pneumocystis carinii enzymology
Substrate Specificity
Genetic Engineering methods
RNA Splicing
RNA, Catalytic genetics
RNA, Catalytic metabolism

Details

Language :
English
ISSN :
1940-6029
Volume :
848
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
22315082
Full Text :
https://doi.org/10.1007/978-1-61779-545-9_24
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