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Deoxyribonuclease is a potential counter regulator of aberrant neutrophil extracellular traps formation after major trauma.
- Source :
-
Mediators of inflammation [Mediators Inflamm] 2012; Vol. 2012, pp. 149560. Date of Electronic Publication: 2012 Jan 23. - Publication Year :
- 2012
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Abstract
- Introduction: Neutrophil extracellular traps (NET) consist of a DNA scaffold that can be destroyed by Deoxyribonuclease (DNase). Thus DNases are potential prerequisites for natural counter regulation of NETs formation. In the present study, we determined the relationship of NETs and DNase after major trauma.<br />Methods: Thirty-nine major trauma patients, 14 with and 25 without sepsis development were enrolled in this prospective study. Levels of cell-free (cf)-DNA/NETs and DNase were quantified daily from admission until day 9 after admission.<br />Results: Levels of cf-DNA/NETs in patients who developed sepsis were significantly increased after trauma. In the early septic phase, DNase values in septic patients were significantly increased compared to patients without sepsis (P < 0.05). cf-DNA/NETs values correlated to values of DNase in all trauma patients and patients with uneventful recovery (P < 0.01) but not in septic patients. Recombinant DNase efficiently degraded NETs released by stimulated neutrophils in a concentration-dependent manner in vitro.<br />Conclusions: DNase degrades NETs in a concentration-dependent manner and therefore could have a potential regulatory effect on NET formation in neutrophils. This may inhibit the antibacterial effects of NETs or protect the tissue from autodestruction in inadequate NETs release in septic patients.
- Subjects :
- Adolescent
Adult
Aged
DNA chemistry
Female
Humans
Inflammation pathology
Inflammation physiopathology
Macromolecular Substances chemistry
Male
Middle Aged
Neutrophils cytology
Prospective Studies
Sepsis immunology
Sepsis microbiology
Sepsis physiopathology
Wounds and Injuries pathology
Wounds and Injuries physiopathology
Young Adult
DNA immunology
Deoxyribonuclease I metabolism
Inflammation immunology
Macromolecular Substances immunology
Neutrophils immunology
Wounds and Injuries immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1861
- Volume :
- 2012
- Database :
- MEDLINE
- Journal :
- Mediators of inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 22315507
- Full Text :
- https://doi.org/10.1155/2012/149560