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Upregulation of UCP2 by adiponectin: the involvement of mitochondrial superoxide and hnRNP K.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (2), pp. e32349. Date of Electronic Publication: 2012 Feb 16. - Publication Year :
- 2012
-
Abstract
- Background: The adipocyte-derived hormone adiponectin elicits protective functions against fatty liver diseases and hepatic injuries at least in part by stimulating the expression of a mitochondrial inner membrane transporter, uncoupling protein 2 (UCP2). The present study was designed to investigate the cellular and molecular mechanisms underlying adiponectin-induced UCP2 expression.<br />Methodology/principal Findings: Mice were treated with adiponectin and/or different drug inhibitors. Parenchymal (PCs) and nonparenchymal (NPCs) cells were fractionated from the liver tissues for mitochondria isolation, Western blotting and quantitative PCR analysis. Mitochondrial superoxide production was monitored by MitoSOX staining and flow cytometry analysis. Compared to control mice, the expression of UCP2 was significantly lower in NPCs, but not PCs of adiponectin knockout mice (AKO). Both chronic and acute treatment with adiponectin selectively increased the mRNA and protein abundance of UCP2 in NPCs, especially in the enriched endothelial cell fractions. The transcription inhibitor actinomycin D could not block adiponectin-induced UCP2 expression, whereas the protein synthesis inhibitor cycloheximide inhibited the elevation of UCP2 protein but not its mRNA levels. Mitochondrial content of heterogeneous nuclear ribonucleoprotein K (hnRNP K), a nucleic acid binding protein involved in regulating mRNA transportation and stabilization, was significantly enhanced by adiponectin, which also evoked a transient elevation of mitochondrial superoxide. Rotenone, an inhibitor of mitochondrial respiratory complex I, abolished adiponectin-induced superoxide production, hnRNP K recruitment and UCP2 expression.<br />Conclusions/significance: Mitochondrial superoxide production stimulated by adiponectin serves as a trigger to initiate the translocation of hnRNP K, which in turn promotes UCP2 expressions in liver.
- Subjects :
- Animals
Endothelial Cells metabolism
Liver cytology
Mice
Mice, Knockout
RNA, Messenger analysis
Uncoupling Protein 2
Up-Regulation
Adiponectin physiology
Heterogeneous-Nuclear Ribonucleoprotein K metabolism
Ion Channels biosynthesis
Mitochondria, Liver metabolism
Mitochondrial Proteins biosynthesis
Superoxides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22359684
- Full Text :
- https://doi.org/10.1371/journal.pone.0032349