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Differentiation and recruitment of Th9 cells stimulated by pleural mesothelial cells in human Mycobacterium tuberculosis infection.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (2), pp. e31710. Date of Electronic Publication: 2012 Feb 20. - Publication Year :
- 2012
-
Abstract
- Newly discovered IL-9-producing CD4(+) helper T cells (Th9 cells) have been reported to contribute to tissue inflammation and immune responses, however, differentiation and immune regulation of Th9 cells in tuberculosis remain unknown. In the present study, our data showed that increased Th9 cells with the phenotype of effector memory cells were found to be in tuberculous pleural effusion as compared with blood. TGF-β was essential for Th9 cell differentiation from naïve CD4(+) T cells stimulated with PMA and ionomycin in vitro for 5 h, and addition of IL-1β, IL-4 or IL-6 further augmented Th9 cell differentiation. Tuberculous pleural effusion and supernatants of cultured pleural mesothelial cells were chemotactic for Th9 cells, and this activity was partly blocked by anti-CCL20 antibody. IL-9 promoted the pleural mesothelial cell repairing and inhibited IFN-γ-induced pleural mesothelial cell apoptosis. Moreover, pleural mesothelial cells promoted Th9 cell differentiation by presenting antigen. Collectively, these data provide new information concerning Th9 cells, in particular the collaborative immune regulation between Th9 cells and pleural mesothelial cells in human M. tuberculosis infection. In particular, pleural mesothelial cells were able to function as antigen-presenting cells to stimulate Th9 cell differentiation.
- Subjects :
- Adult
Antigen Presentation drug effects
Antigen Presentation immunology
Antigens, Bacterial immunology
Apoptosis drug effects
Cell Movement drug effects
Chemokine CCL20 pharmacology
Epithelial Cells drug effects
Epithelial Cells microbiology
Epithelial Cells pathology
Humans
Interferon-gamma pharmacology
Interleukin-4 pharmacology
Ionomycin pharmacology
Lymphocyte Activation drug effects
Middle Aged
Mycobacterium tuberculosis drug effects
Mycobacterium tuberculosis immunology
Mycobacterium tuberculosis physiology
Phenotype
Pleural Effusion immunology
Pleural Effusion microbiology
Pleural Effusion pathology
Receptors, Chemokine metabolism
T-Lymphocytes, Helper-Inducer drug effects
Tetradecanoylphorbol Acetate pharmacology
Tuberculosis microbiology
Tuberculosis pathology
Wound Healing drug effects
Young Adult
Cell Differentiation drug effects
Epithelial Cells immunology
Interleukin-9 immunology
Lymphocyte Activation immunology
Pleura pathology
T-Lymphocytes, Helper-Inducer immunology
Tuberculosis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22363712
- Full Text :
- https://doi.org/10.1371/journal.pone.0031710