Back to Search
Start Over
5-HT7R/G12 signaling regulates neuronal morphology and function in an age-dependent manner.
- Source :
-
The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2012 Feb 29; Vol. 32 (9), pp. 2915-30. - Publication Year :
- 2012
-
Abstract
- The common neurotransmitter serotonin controls different aspects of early neuronal differentiation, although the underlying mechanisms are poorly understood. Here we report that activation of the serotonin 5-HT(7) receptor promotes synaptogenesis and enhances synaptic activity in hippocampal neurons at early postnatal stages. An analysis of Gα(12)-deficient mice reveals a critical role of G(12)-protein for 5-HT(7) receptor-mediated effects in neurons. In organotypic preparations from the hippocampus of juvenile mice, stimulation of 5-HT(7)R/G(12) signaling potentiates formation of dendritic spines, increases neuronal excitability, and modulates synaptic plasticity. In contrast, in older neuronal preparations, morphogenetic and synaptogenic effects of 5-HT(7)/G(12) signaling are abolished. Moreover, inhibition of 5-HT(7) receptor had no effect on synaptic plasticity in hippocampus of adult animals. Expression analysis reveals that the production of 5-HT(7) and Gα(12)-proteins in the hippocampus undergoes strong regulation with a pronounced transient increase during early postnatal stages. Thus, regulated expression of 5-HT(7) receptor and Gα(12)-protein may represent a molecular mechanism by which serotonin specifically modulates formation of initial neuronal networks during early postnatal development.
- Subjects :
- Animals
Animals, Newborn
GTP-Binding Protein alpha Subunits, G12-G13 biosynthesis
GTP-Binding Protein alpha Subunits, G12-G13 genetics
Hippocampus growth & development
Male
Mice
Mice, Inbred C57BL
Organ Culture Techniques
Receptors, Serotonin biosynthesis
Receptors, Serotonin genetics
Synapses genetics
Aging genetics
GTP-Binding Protein alpha Subunits, G12-G13 physiology
Hippocampus cytology
Hippocampus physiology
Neurogenesis genetics
Neurons physiology
Receptors, Serotonin physiology
Signal Transduction genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1529-2401
- Volume :
- 32
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 22378867
- Full Text :
- https://doi.org/10.1523/JNEUROSCI.2765-11.2012