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[The differentiation of oval cells into hepatocytes during induced hepatic carcinogenesis in mice. An electron microscopic study].
- Source :
-
Tsitologiia [Tsitologiia] 1990; Vol. 32 (4), pp. 331-6. - Publication Year :
- 1990
-
Abstract
- An electron microscopic study of murine oval cells, induced by a single injection of genotoxic agent dipin and by a partial hepatectomy, has shown that their ultrastructure and direction of differentiation depend on localization in the liver lobule. Oval cells around portal tracts go through three stages of development: low differentiated cells 4.40 +/- 0.51 mu in diameter with ovoid nuclei 3.43 +/- 0.44 mu, intermediate cells, and young hepatocytes. They form common ducts surrounded by a basal lamina, and produce bile canaliculi-like structures and intermediate junctions between them. Another part of the oval cell population is organized similar to the bile duct epithelium. It consists of cells 9.37 +/- 1.1 mu in diameter with nuclei 7.28 +/- 1.16 mu in diameter and form a system of branching and anastomosing ducts widespread along the parenchyma from the portal to the central veins. Our data indicate that the oval cells can differentiate into hepatocytes, and support a hypothesis according to which the cells of terminal bile ductules are liver epithelial stem cells which can differentiate into a hepatocyte or a bile duct cell lineage in periportal microenvironment.
- Subjects :
- Animals
Aziridines
Carcinogens
Cell Transformation, Neoplastic drug effects
Cell Transformation, Neoplastic ultrastructure
Hepatectomy
Liver drug effects
Liver Neoplasms, Experimental chemically induced
Male
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Microscopy, Electron
Liver ultrastructure
Liver Neoplasms, Experimental ultrastructure
Subjects
Details
- Language :
- Russian
- ISSN :
- 0041-3771
- Volume :
- 32
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Tsitologiia
- Publication Type :
- Academic Journal
- Accession number :
- 2238107