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Berberine suppresses the TPA-induced MMP-1 and MMP-9 expressions through the inhibition of PKC-α in breast cancer cells.
- Source :
-
The Journal of surgical research [J Surg Res] 2012 Jul; Vol. 176 (1), pp. e21-9. Date of Electronic Publication: 2011 Dec 30. - Publication Year :
- 2012
-
Abstract
- Background: Berberine (BBR) is one of the major alkaloids, and it has been reported to have a variety of pharmacologic effects, including inhibition of cell cycle progression. Here, we investigated the effect of BBR on the MMP-1 and MMP-9 expressions, which are predictors of metastasis and invasion in breast cancer cells.<br />Methods: MMP-1 and MMP-9 mRNA expressions were analyzed by real-time PCR. The levels of MMP-1 protein and PKC-α phosphorylation were detected by Western blotting. MMP-9 protein expression was detected by gelatin zymography. Cell cycle was analyzed by FACS analysis. PKC-α knock-down was examined by PKC-α siRNA transfection.<br />Results: The basal levels of both the MMP-1 and MMP-9 mRNA expressions were decreased by BBR treatment in a dose-dependent manner. In contrast, TPA, which is a tumor promoter, significantly increased the levels of the MMP-1 and MMP-9 mRNA and protein expressions in the MCF-7 breast cancer cells. We also observed that the TPA-induced MMP-1 and MMP-9 mRNA and protein expressions were prevented by BBR treatment. In addition, the TPA-induced MMP-1 and MMP-9 expressions were completely decreased by Go6983 and PKC-α siRNA, respectively. TPA-induced PKC-α phosphorylation was dose-dependently decreased by BBR treatment.<br />Conclusion: The TPA-induced PKC-α phosphorylation is suppressed and then the MMP-1 and MMP-9 expressions are also inhibited by berberine. Therefore, we suggest that berberine may be used as a candidate drug for the inhibition of metastasis of human breast cancer.<br /> (Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adenocarcinoma metabolism
Adenocarcinoma pathology
Breast Neoplasms pathology
Carcinogens pharmacology
Cell Line, Tumor
Cell Movement drug effects
Dose-Response Relationship, Drug
Female
Humans
Phosphorylation drug effects
Protein Kinase C-alpha metabolism
RNA, Messenger metabolism
Berberine pharmacology
Breast Neoplasms metabolism
Matrix Metalloproteinase 1 metabolism
Matrix Metalloproteinase 9 metabolism
Protein Kinase C-alpha antagonists & inhibitors
Tetradecanoylphorbol Acetate pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8673
- Volume :
- 176
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of surgical research
- Publication Type :
- Academic Journal
- Accession number :
- 22381172
- Full Text :
- https://doi.org/10.1016/j.jss.2011.11.1041