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Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor.
- Source :
-
Journal of translational medicine [J Transl Med] 2012 Mar 13; Vol. 10, pp. 46. Date of Electronic Publication: 2012 Mar 13. - Publication Year :
- 2012
-
Abstract
- The testicular yolk sac tumor (TYST) is the most common neoplasm originated from germ cells differentiated abnormally, a major part of pediatric malignant testicular tumors. The present study aimed at developing and validating the in vitro and vivo models of TYST and evaluating the sensitivity of TYST to treatments, by cloning human TYST cells and investigating the histology, ultra-structure, growth kinetics and expression of specific proteins of cloned cells. We found biological characteristics of cloned TYST cells were similar to the yolk sac tumor and differentiated from the columnar to glandular-like or goblet cells-like cells. Chromosomes for tumor identification in each passage met nature of the primary tumor. TYST cells were more sensitive to all-trans-retinoic acid which had significantly inhibitory effects on cell proliferation. Cisplatin induced apoptosis of TYST cells through the activation of p53 expression and down-regulation of Bcl- expression. Thus, we believe that cloned TYST cells and the animal model developed here are useful to understand the molecular mechanism of TYST cells and develop potential therapies for human TYST.<br /> (© 2012 Zhao et al; licensee BioMed Central Ltd.)
- Subjects :
- Animals
Cell Cycle drug effects
Cell Proliferation drug effects
Child, Preschool
Cisplatin pharmacology
Clone Cells
Endodermal Sinus Tumor pathology
Endodermal Sinus Tumor ultrastructure
Humans
Male
Mice
Mice, Inbred BALB C
Neoplasm Proteins metabolism
Testicular Neoplasms pathology
Testicular Neoplasms ultrastructure
Tretinoin pharmacology
Tumor Burden drug effects
alpha-Fetoproteins metabolism
Endodermal Sinus Tumor drug therapy
Testicular Neoplasms drug therapy
Tretinoin therapeutic use
Xenograft Model Antitumor Assays
Subjects
Details
- Language :
- English
- ISSN :
- 1479-5876
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of translational medicine
- Publication Type :
- Academic Journal
- Accession number :
- 22410253
- Full Text :
- https://doi.org/10.1186/1479-5876-10-46