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Immunotherapy with oligomannose-coated liposomes ameliorates allergic symptoms in a murine food allergy model.
- Source :
-
Allergy [Allergy] 2012 Mar; Vol. 67 (3), pp. 371-9. - Publication Year :
- 2012
-
Abstract
- Background: Allergen-specific immunotherapy has been anticipated to be a disease-modifying therapy for food allergies. We previously reported that CD8(+) regulatory T cells may prevent antigen-sensitized mice from developing allergic diarrhea. Because oligomannose-coated liposomes (OML) have been shown to induce MHC class I-restricted CD8(+) T cell responses, we analyzed the adjuvant activities of OML for inducing regulatory CD8(+) T cells and mucosal tolerogenic responses in allergen-sensitized mice.<br />Methods: The BALB/c mice that were previously sensitized to ovalbumin (OVA) were intranasally immunized with OVA-encased in OML (OVA-OML) or OVA-encased in non-coated liposomes (OVA-NL). We assessed allergic diarrhea induced by oral OVA administration, OVA-specific immunoglobulin production, and cytokine production in the intestines and mesenteric lymph nodes (MLNs).<br />Results: Intranasal immunization with OVA-OML, but not OVA-NL, suppressed the development of allergic diarrhea. This was associated with in vitro Ag-induced IL-10 production and the in vivo expansion of CD8(+) CD28(-) and CD4(+) CD25(+) Foxp3(+) T cell populations among mesenteric lymph node mononuclear cells, and was significantly ablated by anti-SIGNR1 or anti-CR3 mAbs. Up-regulation of serum OVA-specific IgE was suppressed, whereas OVA-specific IgG1, IgG2a, and soluble IgA production were enhanced by intranasal administration of OVA-OML. Adoptive transfer of CD8(+) CD28(-) T cells but not CD28(+) CD8(+) T cells from the MLNs of OVA-OML-treated mice ameliorated the development of diarrhea.<br />Conclusion: These results suggest that intranasal immunization with Ag-encased OML may be an effective immunotherapy for food allergies, as it induces a subset of regulatory CD8(+) T cells as well as CD4(+) CD25(+) Foxp3(+) T cell and modulates humoral immune responses in allergen-sensitized mice.
- Subjects :
- Animals
CD8-Positive T-Lymphocytes immunology
Diarrhea therapy
Food Hypersensitivity immunology
Humans
Liposomes immunology
Mice
Mice, Inbred BALB C
Oligosaccharides immunology
Ovalbumin immunology
Ovalbumin therapeutic use
Treatment Outcome
Desensitization, Immunologic methods
Disease Models, Animal
Food Hypersensitivity therapy
Liposomes therapeutic use
Oligosaccharides therapeutic use
T-Lymphocytes, Regulatory immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1398-9995
- Volume :
- 67
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 22423374
- Full Text :
- https://doi.org/10.1111/j.1398-9995.2011.02777.x