Back to Search
Start Over
Involvement of CtBP2 in LPS-induced microglial activation.
- Source :
-
Journal of molecular histology [J Mol Histol] 2012 Jun; Vol. 43 (3), pp. 327-34. Date of Electronic Publication: 2012 Mar 17. - Publication Year :
- 2012
-
Abstract
- CtBP2 (C-terminal binding protein 2), which is widely expressed during developmental processes and differentiation, acts as a transcriptional repressor following recruitment to target promoters through repressors or other co-repressor proteins. In this study, we elucidated the dynamic expression changes and localization of CtBP2 in lipopolysaccharide (LPS)-induced neuroinflammatory processes in adult rats. CtBP2 expression was strongly induced in active glia cells (microglia and astrocytes) in inflamed spinal cord. In vitro studies indicated that the up-regulation of CtBP2 may be involved in the subsequent microglia activation following LPS exposure. And the knock-down of CtBP2 in microglia cell line HAPI by siRNA showed that CtBP2 increased the activation of microglia induced by LPS. Collectively, these results suggested CtBP2 may be important in host defense in microglia-mediated immune response. Understanding the cell signal pathway may provide a novel strategy against inflammatory and immune reaction in neuroinflammation in central nervous system.
- Subjects :
- Animals
Astrocytes metabolism
Astrocytes pathology
Blotting, Western
Cell Line
Eye Proteins antagonists & inhibitors
Eye Proteins metabolism
Gene Expression drug effects
Gene Silencing
Inflammation chemically induced
Inflammation genetics
Injections, Spinal
Lipopolysaccharides administration & dosage
Microglia metabolism
Microglia pathology
RNA, Small Interfering genetics
Rats
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Spinal Cord metabolism
Spinal Cord pathology
Transfection
Astrocytes drug effects
Eye Proteins genetics
Inflammation metabolism
Microglia drug effects
Spinal Cord drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1567-2387
- Volume :
- 43
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of molecular histology
- Publication Type :
- Academic Journal
- Accession number :
- 22426895
- Full Text :
- https://doi.org/10.1007/s10735-012-9399-x