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Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections.
- Source :
-
Proteome science [Proteome Sci] 2012 Mar 23; Vol. 10, pp. 19. Date of Electronic Publication: 2012 Mar 23. - Publication Year :
- 2012
-
Abstract
- We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR.
Details
- Language :
- English
- ISSN :
- 1477-5956
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- Proteome science
- Publication Type :
- Academic Journal
- Accession number :
- 22439923
- Full Text :
- https://doi.org/10.1186/1477-5956-10-19