Back to Search
Start Over
Flow cytometric chromosome sorting in plants: the next generation.
- Source :
-
Methods (San Diego, Calif.) [Methods] 2012 Jul; Vol. 57 (3), pp. 331-7. Date of Electronic Publication: 2012 Mar 14. - Publication Year :
- 2012
-
Abstract
- Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Chromosomes, Artificial, Bacterial
Flow Cytometry methods
Genome Size
Germination genetics
High-Throughput Nucleotide Sequencing
Hordeum cytology
In Situ Hybridization, Fluorescence
Metaphase genetics
Microscopy, Fluorescence
Polyploidy
Secale cytology
Sequence Analysis, DNA
Triticum cytology
Chromosomes, Plant ultrastructure
Genome, Plant
Hordeum genetics
Secale genetics
Seeds genetics
Triticum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9130
- Volume :
- 57
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Methods (San Diego, Calif.)
- Publication Type :
- Academic Journal
- Accession number :
- 22440520
- Full Text :
- https://doi.org/10.1016/j.ymeth.2012.03.006