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Oligopeptides functionalized surface plasmon resonance biosensors for detecting thiacloprid and imidacloprid.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2012 May 15; Vol. 35 (1), pp. 271-276. Date of Electronic Publication: 2012 Mar 07. - Publication Year :
- 2012
-
Abstract
- By using phage display library, we identified two highly specific oligopeptide sequences RKRIRRMMPRPS and RNRHTHLRTRPR for binding neonicotinoids such as thiacloprid and imidacloprid. The former shows high affinity for thiacloprid whereas the latter shows high affinity for imidacloprid. Surprisingly, cross binding is minimal despite the similarity of the two molecules. To develop a neonicotinoid biosensor, these two oligopeptides are synthesized and immobilized on the surface of a surface plasmon resonance (SPR) chip with a bare-gold surface. This oligopeptide functionalized SPR biosensor can rapidly detect thiacloprid and imidacloprid in buffer solutions in a real-time manner. The limit of detection (LOD) for thiacloprid and imidacloprid is 1.2 μM and 0.9 μM, respectively.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Immobilized Proteins chemistry
Limit of Detection
Neonicotinoids
Nicotine analogs & derivatives
Nicotine analysis
Peptide Library
Protein Binding
Receptors, Nicotinic metabolism
Surface Plasmon Resonance statistics & numerical data
Imidazoles analysis
Nitro Compounds analysis
Oligopeptides chemistry
Pyridines analysis
Surface Plasmon Resonance methods
Thiazines analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 22459587
- Full Text :
- https://doi.org/10.1016/j.bios.2012.02.060