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Suppression of astrocyte lineage in adult hippocampal progenitor cells expressing hippocampal cholinergic neurostimulating Peptide precursor in an in vivo ischemic model.
- Source :
-
Cell transplantation [Cell Transplant] 2012; Vol. 21 (10), pp. 2159-69. Date of Electronic Publication: 2012 Mar 26. - Publication Year :
- 2012
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Abstract
- Hippocampal cholinergic neurostimulating peptide (HCNP) is known to promote differentiation of septohippocampal cholinergic neurons. The HCNP precursor protein (HCNP-pp) may play several roles, for example, as an ATP-binding protein, a Raf kinase inhibitor protein, and a phosphatidylethanolamine-binding protein, as well as a precursor for HCNP. This study therefore aimed to elucidate the involvement of HCNP-pp in specific neural lineages after stroke using a hypoxic-ischemic (HI) rat model of brain ischemia. The specific neural lineages in the hippocampus were investigated 14 days after ischemia. Some bromodeoxyuridine (BrdU)(+) neural progenitor cells in the hippocampus of hypoxic, HI, or sham-operated rats expressed HCNP-pp. Almost half of the BrdU(+)/HCNP-pp(+) cells also expressed the oligodendrocyte lineage marker 2',3'-cyclic nucleotide 3'-phosphodiesterase, whereas only a few BrdU(+)/HCNP-pp(+) cells in the hippocampus in HI brains expressed the neuronal lineage marker, doublecortin (DCX). Interestingly, no BrdU(+)/HCNP-pp(+) progenitor cells in hypoxic, HI, or sham-operated brains expressed the astrocyte lineage marker, glial fibrillary acidic protein. Together with previous in vitro data, the results of this study suggest that the expression level of HCNP-pp regulates the differentiation of neural progenitor cells into specific neural lineages in the HI hippocampus, indicating that neural stem cell fate can be controlled via the HCNP-pp mediating pathway.
- Subjects :
- Animals
Astrocytes metabolism
Brain Ischemia metabolism
Cell Differentiation physiology
Cell Growth Processes physiology
Cell Lineage
Disease Models, Animal
Doublecortin Protein
Female
Immunohistochemistry
Male
Neural Stem Cells metabolism
Rats
Rats, Sprague-Dawley
Astrocytes cytology
Brain Ischemia pathology
Neural Stem Cells cytology
Phosphatidylethanolamine Binding Protein biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1555-3892
- Volume :
- 21
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cell transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 22469272
- Full Text :
- https://doi.org/10.3727/096368911X627543