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[Effect of monocytes activated with lipopolysaccharide on human Th17 cell differentiation].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2012 Apr; Vol. 28 (4), pp. 377-80. - Publication Year :
- 2012
-
Abstract
- Aim: To investigate whether monocytes activated with lipopolysaccharide(LPS) have an effect on Th17 cell differentiation in humans, CD4(+) T cell and CD14(+) monocytes activated with LPS were treated in the absence or presence of anti-CD3 mAb with various concentrations at different time points.<br />Methods: Purification of CD4(+) T cell and CD14(+) monocytes were performed by magnetic cell sorting and cultured together. Cultures were stimulated with LPS alone or anti-CD3 mAb alone or LPS plus anti-CD3 mAb for 3 days. In the anti-CD3 mAb stimulation cells were added different concentrations of LPS. Cells were activated under LPS/anti-CD3 costimulation for 3, 6, or 10 days. The percentage of IL-17(+) T cells and INF-γ(+) T was determined by flow cytometry.<br />Results: LPS or anti-CD3 mAb alone induced only very low levels of IL-17(+) T cells, (1.30 ± 0.19)%, (1.10 ± 0.21)%, respectively. The percentage was substantially higher in the LPS and anti-CD3 mAb costimulationa as much as(2.01 ± 0.46)%. In the presence of 0.1 μg/mL, 1 μg/mL, 10 μg/mL LPS, the proportion of Th17 reached to (1.92 ± 0.21)%, (1.30 ± 0.37)%, (1.01 ± 0.25)%. Low-concentration LPS (0.1 μg/mL) stimulation favored Th17 differentiation. The highest proportion of IL-17(+) T cells was found at day 3(2.13 ± 0.32)%, with levels declining at day 6 and day 10, while, Th1 at day 6(17.45 ± 3.04)%, declining at day 10.<br />Conclusion: Low-concentration LPS stimulation plus anti-CD3 mAb in short term support optimal Th17 generation. Nevertheless, this model closely mimics the environment of rheumatoid arthritis in vivo and proposes an effective model for the generation of human Th17 cells.
- Subjects :
- Antibodies, Monoclonal pharmacology
CD3 Complex immunology
CD4-Positive T-Lymphocytes cytology
CD4-Positive T-Lymphocytes metabolism
Cell Differentiation drug effects
Cells, Cultured
Coculture Techniques
Dose-Response Relationship, Drug
Flow Cytometry
Humans
Interferon-gamma immunology
Interferon-gamma metabolism
Interleukin-17 immunology
Interleukin-17 metabolism
Lipopolysaccharide Receptors immunology
Lipopolysaccharide Receptors metabolism
Lipopolysaccharides pharmacology
Lymphocyte Activation drug effects
Lymphocyte Activation immunology
Monocytes cytology
Monocytes metabolism
Th17 Cells cytology
Th17 Cells metabolism
Time Factors
CD4-Positive T-Lymphocytes immunology
Cell Differentiation immunology
Monocytes immunology
Th17 Cells immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 22482408