Quantification by real-time PCR assay of Staphylococcus aureus load: a useful tool for rapidly identifying persistent nasal carriers.

Authors :: Verhoeven PO
Grattard F
Carricajo A
Lucht F
Cazorla C
Garraud O
Pozzetto B
Berthelot P
Source :: Journal of clinical microbiology [J Clin Microbiol] 2012 Jun; Vol. 50 (6), pp. 2063-5. Date of Electronic Publication: 2012 Apr 04.
Publication Year :: 2012
Abstract: The Cepheid Xpert MRSA/SA nasal PCR assay was compared to culture for quantifying Staphylococcus aureus load from 104 nasal samples (r = 0.91, P < 0.0001). Using a bacterial load-based algorithm, the test was found able to predict the carrier state in 32 of 35 healthy volunteers (22 persistent and 13 nonpersistent carriers).

Subjects :: Algorithms
Humans
Methicillin-Resistant Staphylococcus aureus genetics
Sensitivity and Specificity
Bacterial Load methods
Carrier State microbiology
Methicillin-Resistant Staphylococcus aureus isolation & purification
Nose microbiology
Real-Time Polymerase Chain Reaction methods
Staphylococcal Infections microbiology

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